What is the cause of the banding obtained after purifying the PCR product with ExoSAPIT?

Hello We are working on a PCR product with an approximate size between 5000 and 7000 bp, we want to use it as a positive internal control, since we have sequenced it and it corresponds to the...

17 February 2021 2,896 3 View

CAT assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!

11 February 2021 7,273 3 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

Any good reference that links economic/industrial policy to changed agent behavior?

Usually industrial policy is interpreted as changing economic structure from in favor of more productive/advanced economic activities. But would like to read something that discusses the...

31 December 2020 3,199 3 View

How to calculate the necessary sample size for a subgroup descriptive analysis?

I want to do a descriptive analysis using the World Values Survey dataset which has an N=1200. However, even thought I have searched a lot, I haven't found the methodology or a tool to calculate...

17 November 2020 1,505 4 View

What is the relationship between plant hormones and plant allometry?

Hi, i'm looking for bibliographic references that explains the relationship between plant hormones and plant allometry (relation between the size of an organism and aspects of its physiology and...

21 September 2020 9,653 6 View

Why does the value of fresh and dry weight change in a plant?

In an experiment I found that the fresh weight of the root was less than that of the stem, however when I found the dry weight, the weight of the stem was less than that of the root. Why are these...

20 September 2020 2,269 16 View

Has anynone successfully used NEB's TtAgo (Thermus Thermophilus Argonaute) for any type of application?

I have been trying to use NEB's TtAgo (https://www.neb.com/products/m0665-ttago) to linearize supercoiled plasmids, following NEB's recommendations, but I haven't had any success yet (19/19 failed...

09 September 2020 2,112 1 View

What does it really mean to "hold other variables constant" in a regression?

To illustrate my point I present you an hypothetical case with the following equation: wage=C+0.5education+0.3rural area ( Where the variable "education" measures the number of years of...

10 July 2020 2,839 12 View

Is there a NO corrosion application for a linear polarization resistance?

Is there a NO corrosion application for a linear polarization resistance? Any example? And what is the technical justification for using +/- 10 mV as a signal of disturbance in linear...

09 June 2020 3,825 4 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

Can I publish my published dataset (data-report article) later as an original article?

I want to quickly publish a data-report .Because there is a lot of data and it will take time to create the original article. But my main goal is to use that data-set in the original article. Is...

02 February 2021 9,779 7 View

Does anybody has the biodot zx1010 manual?

25 January 2021 6,257 1 View

The > 91 Years long collective IQ Blunder ?

Never before was there a bigger blemish. Namely: Not to collectively underastand Zwicky's discovery of Cryodynamics, as his sister to deterministic Thermodynamics came to be called later. Dec....

30 December 2020 8,300 5 View

Is explanatory sequential design the right design for my research?

I am interested in finding the effects of technology on the learning achievement of students. The data collection will include pre and post test scores of the experiment group and later...

26 December 2020 3,757 4 View

Creating GFP Fusion Protein Using pCAMBIA Vector?

I intend to fuse my gene of interest (apoptin) with GFP using pCAMBIA1302 vector. I figured that I can do that by adding NcoI and SpeI restriction sites to 5' and 3' end of my GOI using PCR and...

13 December 2020 4,335 3 View

What can I use to dilute protein for gelatin zymography?

My protein sample is extracted with RIPA + PMSF ( Phenylmethylsulfonyl fluoride). After protein assay, the concentration is too high (>900ug/mL) for direct application for gelatin zymography...

13 December 2020 7,764 3 View

Who is to be cited in the research/review articles?

When an author want to cite the work/idea/theoretical model/results/facts for publication purpose, who should s/he cited (giving citation credit)? (a) the first author (pioneer) who...

09 November 2020 6,929 3 View

How can i limit the discharging energy from the EV in an Energy management system?

Hi everyone, i am making optimization for energy management system of EV charging station. I established the mathematical model objective function (minimization of electricity cost) and...

28 October 2020 7,267 3 View