Hi Shahadat,

To my best awareness, such protocols do not currently exist per se. But the technology that you would need to perform such recordings does.

Doing the experiment that you would like to do is by no means easy, but I would recommend you start by looking at using iGluSnFR as your sensor (see, e.g., www.ncbi.nlm.nih.gov/pubmed/23314171 for the initial sensor description and http://www.jneurosci.org/content/36/4/1261 for an example use of iGluSnFR in vivo).

In contrast the work by Xie et al., you'll be working below the depth where standard 1- and 2-photon imaging modalities are capable of operating. But you could use something similar to the system recently developed by Deisseroth's group (http://www.nature.com/nmeth/journal/v13/n4/abs/nmeth.3770.html) to simultaneously image field glutamate levels in both of your structures of interest. Incidentally, the isobestic normalization trick they use would also work for iGluSnFR.

Please feel free to message me if you have any further questions on the topic.

Best,

Joey

https://www.ncbi.nlm.nih.gov/pubmed/23314171

http://www.jneurosci.org/content/36/4/1261

http://www.nature.com/nmeth/journal/v13/n4/abs/nmeth.3770.html

How about use microdialysis and detect it offline with HPLC.