Could expired BSA a problem for tissue culture?

Hi, I want to ask if for you using expired BSA powder (expired in 2014 but always in the fridge and protected from light) could be a problem for my bovine primary follicles culture in situ (so...

01 December 2020 8,650 2 View

Neyman - Scott Cluster Process: are these nonsensical parameter values ?

I am trying to fit a cluster model in spatstat in R using the locations of archaeological sites. The resulting parameters values are: trend: 2.514429e-06 kappa: 1.606992e-08 scale: 481.5851 mu:...

18 November 2020 2,993 3 View

Flow cytometry from harvested non-uniform tissue?

Hi everyone! I want to identify by flow cytometry the immune cells found in the brain with a localized injury. My hypotesis is that the injury will attract more/different immune cells respect to...

27 October 2020 748 2 View

What do you use to avoid insect collection infestation?

At the museum we are currently dismissing Paradichlorobenzene and Naphtalene. we are looking for other effective chemical or similar.

21 October 2020 9,553 11 View

Issue with plasmid cloning?

22 August 2020 1,687 15 View

Is there any commercial or open source DIC (2D or 3D) software which is capable of working on field photographs or satellite images?

Is there any commercial or open-source DIC (2D or 3D) software which is capable of working on field photographs of terrain or satellite images for deformation and strain analysis in geophysical...

11 August 2020 4,216 3 View

How to perform in silico peptide cyclization reactions by ChemAxon Reactor?

24 April 2020 9,398 1 View

Does the EGFP epression in a BAC-EGFP mouse correlate with the amount of promoter gene expression?

Hi, I am analyzing immunostainings of 5-HT2AR-EGFP transgenic mice (Htr2a Gene and 5-HT2A Receptor Expression in the Cerebral Co... ) and I have a doubt regarding the conclusion I can draw about...

21 July 2019 209 1 View

Is it possible to "paste" two functions in COMSOL?

Hi everyone, here is the description of the problem. I have a rectangle with some circular holes in it, the inner part of the domain (denoted with D) is defined by the difference between the...

07 July 2019 1,635 3 View

How to avoid for order effects in an experiment with 20 participants and one task with 12 trials?

Hey, we are studying the relationship between trait anxiety and spatial navigation. We have 20 participants and one navigation task with 12 trials. How to avoid for order effects among the trials?...

07 April 2019 9,479 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View