Does anyone have insights on the presence and functional characterization of SECIS elements and selenoproteins in s.coelicolor?

05 November 2024 0 7K Report

I am currently conducting a comparative analysis of selenocysteine biosynthesis potential in Streptomyces species, with a focus on selenoproteins SelA, SelB, and SelD. I’m using the Galaxy platform for my bioinformatics workflow, but am finding it challenging to confirm specific annotations related to selenoproteins in S. coelicolor. If anyone has experience with verifying SECIS elements or has encountered similar issues with Galaxy, I would be grateful for advice on complementary tools or strategies.

Badges
Science topic

Similar topics
Chemistry
Carbon

More Soniya Singh's questions See All

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How to enrich pig excreta for increasing nutrient quality organically ?

Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.

09 August 2024 5,605 2 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Leaf area of tomato ?

Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?

06 August 2024 2,508 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

How to increase citation in Research Gate?

How to enhanced h-index in Research Gate?

04 August 2024 3,368 4 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

Is Galaxy.org good to use for research for analyzing data and for publication?

Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)

06 August 2024 6,610 4 View

How can biogenic synthesis techniques be applied to develop plants capable of extracting and processing heavy metals from contaminated soil, and wha?

Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.

26 July 2024 1,469 1 View

What is wrong about this model of Universe?

A universe model compatible with VSLT The research of Halton Arp and Eric Lerner supports a stationary universe, and the intrinsic nature of redshift, i.e. not linked to its presumed expansion...

14 July 2024 4,092 5 View

When Streptomyces undergoes conjugation transfer, it is always contaminated by a type of yellow Pseudomonas. What could be the reason for this?

07 July 2024 5,480 2 View

What is the effect of redshift on the evolution of an AGN?

AGN (Active Galactic Nuclei) are high energy galaxies powered by supermassive blackholes. The evolution of a galaxy is characterized by parameters such as luminosity and redshift. The redshift is...

25 June 2024 3,782 2 View

How to design primers and run PCR in SerialCloner?

I am working on a paper for my bioinformatics class, where we are running an analysis of transcriptomics on A. thaliana col-0 about the difference in gene expression in the temperatures of 21 and...

12 June 2024 8,822 1 View

How to calculate the mass of an elliptical galaxy?

Hello, I would like to know how to calculate the mass of an elliptical galaxy. If someone could explain the commonly used methods and formulas, it would be greatly appreciated. Additionally, could...

02 June 2024 3,239 10 View

I am Extracting Streptomyces DNA with CTAB method. I would like to know When should I add RNAse in the CTAB protocol for Streptomyces DNA extraction?

I did CTAB for DNA extraction and I got good DNA IN BOTH side (Quantity and Quality) but I had RNA in my gel. I wanted to add RNAse after that and when I add it my DNA were degraded. Could you...

18 April 2024 3,595 0 View

How do you prepare a solid sample of biosynthesized Silver Nanoparticle (AgNP) for a UV-Vis spectrophotometer?

I have synthesized AgNP prepared from a plant leaf extract and AgNO3 solution. It underwent optimization by applying different parameters (reaction time, pH, concentration, etc). The solid NPs...

12 April 2024 6,042 2 View

How can I optimize the pH of biosynthesis AgNP? Is it acceptable to use chemical reagents to adjust the pH in green synthesis approaches?

I am planning to synthesize AgNP using plant extract as a bioreductor. I have come across several papers recommending a neutral to alkaline pH range (pH 7-8) to achieve silver nanoparticles with...

19 March 2024 1,246 4 View