Dear Scientists,
Does anyone have any suggestions or information about performing "Dose determination" of selectable markers Neomycin & Hygromycin for Human cell lines?
Best Regards,
Manizheh Narimani
Ph.D Candidate of Molecular Medicine
https://www.mirusbio.com/applications/stable-cell-line-generation/antibiotic-kill-curve
I think you are wanting to perform an antibiotic Kill Curve. Please see the website above for directions.
Good luck!
Thanks a lot
I used 15ul (500mg/ml concentration for ampiciline) for each plat
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