What tests for: 1. differences between continuous variables when grouped with different categorical ones; 2. significance of potential relationships?

Hello. I was wondering if anyone had suggestions in terms of what exploratory analysis to carry out for my dissertation with the following data. I measured N=26 participants pre and post an...

09 August 2020 5,256 5 View

Anti-His Antibody binding everything on a western?

Hello all, I've been expressing some His-tagged proteins in E.coli that appears to be going just fine based on how the SDS-PAGE gels look, so I wanted to confirm with a Western. I transferred my...

22 July 2020 5,255 5 View

Is it possible to run a Mediation Analysis with Process when the dependant variable is a percentage value?

I measured the DV by presenting the participants two product options where they had to choose one of them. I repeated this 20 times. After that I calculated the percentage value of choosing the...

05 November 2019 9,978 3 View

How to codify a sequence DNA correctly for DNA barcoding analysis?

Hello, I am starting a new project with DNA barcoding and I am not sure how to codify sequence DNA. In this project we are using the following molecular markers: rps16-trnk, rpl32-trnl and...

26 July 2018 5,797 2 View

Is it possible to impose a cpu limit on executors in Apache storm?

Each executor is a thread inside a worker process. Is it possible to set some rule such that all the executors on a machine execute for (roughly) equal time? so that one executor is not hogging...

12 February 2018 8,355 1 View

How do you do the normalisation of X protein to study the effect of a protein Y mutation on X protein level by western blot?

I need to study the effect of the protein Y mutation on the levels of a protein X. So I over-expressed the protein X under 5 conditions: (1) not over-expression of protein Y (2) with...

07 June 2017 5,252 4 View

Is it possible to load 120 ug of proteins in a protein gel?

I need to load 120 ug of proteins in a protein gel. Do I need some precaution for the protein migration and western-blot?

11 July 2016 624 4 View

Contaminated liquid culture of yeast turns red upon addition of bleach, why ?

The medium is YPAD, and it is probably contaminated with bacteria. I suppose this kind must synthesize some pH sensitive molecules as It is reacting with bleach but I can't find any information on...

09 February 2016 4,101 3 View

High variability in western blot, why?

I am trying to detect endogenous Aurora B in the whole cell extract but I have high variability during the detection by LiCOR scanner. I use 2 different primary antibodies from BD technology and...

10 December 2015 4,007 6 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View