https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229753/

I agree with Dr.Azhar

Dear Meenu, 

there are numerous variations of EAE using different susceptible mouse strains and major or minor myelin antigens and peptides. In addition to the above mentioned review, check out http://www.sciencedirect.com/science/article/pii/S0896627300811071

From my own experience you can try inducing EAE in H2-r expressing B10.RIII mice with MBP protein or the immunodominant MBP peptide 89-101. It will produce a chronic progressing, relapsing disease. For details on the experimental protocol please consider

http://onlinelibrary.wiley.com/doi/10.1002/eji.1830210323/abstract;jsessionid=8DD0829B6D35B1F3DDEE7AD7562DAC7C.f04t03

All the best & good luck with your experiments,

Michael

I used C57Bl/6J mice to study EAE in my old lab, but we used myelin oligoglycodendrocyte protein peptide 35-55. When using this you will achieve a progressing disease approximately 14 days after immunization . We also gave pertussis toxin ip day 0 and 2 to make the BBB more permeable . Be aware that there is no 100% incidence in any experiment so some mice will have to be boosted or thrown out .

Best regards,

Kirstine