I was looking around for a new perfusion pump for mouse transcardial perfusion and fixation of the brain (and subsequent IHC), and came across this Perfusion Two system from Leica which not only seems very convenient (perfusion in seconds) but also claims to prevent shrinkage and be generally more reliable and effective (by using a particular (high) pressure rather than relying on a set flow rate). It would be great if anyone who is not from Leica could share their experiences with this system, compared to a 'standard' pump or even gravity perfusion system!
We had serious problems with air bubbles in the Leica perfusion system, pretty big ones (several ml). The sales man came several times to assist us, but he also couldn't get rid of the problem. Finally we got refunded.
I just checked the homepage, and the product seems unchanged to me, so I would be surprised if they solved the problem.
If you really want to go for it, ask Leica for a trail period...
thank you very much for your input! Anyway we had our doubts about investing so much in comparison to a 'normal' pump, so the trial period sounds like a good idea..
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