In pfa-fixed 70um thick sections, I have neurobiotin-filled cells (spiny, pyramidal-like morphology), mostly in the presubiculum, for which I would like to confirm that they are glutamatergic pyramidal cells using fluorescence microscopy. Two complications:

1. for most cells, only the dendrites and soma are visible, so no axon (terminals).

2. Because of previous reactions done with these sections, I only have one channel left, so I can only use one antibody! (i.e. I cannot use an additional marker to prove that cells are not GABAergic or not glial cells)

Maybe it's just not possible to get a 100% answer, but I'd like to get as close as possible :). Any suggestions very welcome! Eg any opinions on Emx1? Thanks!