John J Tukker

3 Questions 4 Answers 0 Followers

Questions related from John J Tukker

Does anyone have experience with the Leica Perfusion One/Two system(s)?

I was looking around for a new perfusion pump for mouse transcardial perfusion and fixation of the brain (and subsequent IHC), and came across this Perfusion Two system from Leica which not only...

07 July 2017 6,712 2 View

Is there any reason not to mix two AAVs into the same injection? Ie is there a danger of some interaction?

I mixed AAV9 and some borrowed AAV2 (both with EF1a, FLEX and ChR2, but with 2 different fluorophores) into an epi so that I can inject them together, to assess effect of serotype directly at the...

10 October 2015 8,794 11 View

Can anyone recommend a good antibody to confirm glutamatergic pyramidal cell identity, that does not rely on synaptic terminal staining?

In pfa-fixed 70um thick sections, I have neurobiotin-filled cells (spiny, pyramidal-like morphology), mostly in the presubiculum, for which I would like to confirm that they are glutamatergic...

07 July 2014 8,547 1 View