I´m performing existence/non existence assays with real time PCR but I need an IPC blocked (IPC- blocked reagent) for every reaction, does anyone knows how to replace that reagent o what should I do to replace it? Thank you.
It depends on your internal control? Is this an additional template you are adding to each reaction from ABI . .ahh . .Life . . I mean, Thermo ?
Depending on your sample, you might be able to use an endogenous IC but for some of these, we use a BLOCK system as well to chemically cripple the internal control so as not to prevent low level positives from being outcompeted by a high level IC
Thank you. Let me tell you some information about my essays: I´m trying to find a phytopathogen from the floem of plants and because of it my internal positive control (IPC) is the COX gene of the plant DNA (probe labeled with TET). The pathogen DNA probe is labeled with FAM. My IPC is a sample positive to COX and negative to FAM (I mean, a healty plant without the phytopathogen).
The software of the Real time PCR equipment needs the block system that you mentioned (called IPC-/IPC blocked), but I would like to know which is the blocking system?, can I replace it use with another reagent?, or what are their components?
I appreciate your help.
Thank you. Let me tell you some information about my essays: I´m trying to find a phytopathogen from the floem of plants and because of it my internal positive control (IPC) is the COX gene of the plant DNA (probe labeled with TET). The pathogen DNA probe is labeled with FAM. My IPC is a sample positive to COX and negative to FAM (I mean, a healty plant without the phytopathogen).
The software of the Real time PCR equipment needs the block system that you mentioned (called IPC-/IPC blocked), but I would like to know which is the blocking system?, can I replace it use with another reagent?, or what are their components?
I appreciate your help.
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