Does anyone have experience with molecular beacon design?

More Yoav Lubelsky's questions See All

Do dog have sense of humor?

Is there any bioassay for detecting/ evaluating dogs (or other animal) sense of humor?

20 July 2023 8,678 2 View

How can ligand pdbqt input files to AutoDock 4 be batched?

Hi, I am using AutoDock 4 for covalent docking. I wonder - is it possible to provide as input a pdbqt file with multiple ligands included, and later parse the dlg output accordingly? If so - what...

28 June 2023 8,818 3 View

How to monitor citrus rust mite?

Does anybody knows of a systematic monitoring methodology which is indipendet on the skill and the eyes of the observer i.e. any one that monitors will end with same result

21 September 2020 5,700 1 View

What HPLC columns are usable for determining sucrose levels?

I'm trying to understand what kind of HPLC (High Performance Liquid Chromatography) column should I use for quantifying sucrose levels in a solution (extracted from plant material).

13 July 2020 1,775 2 View

How PDMS bonded to SU8 that covers microfabricated gold electrodes affect their electrochemical activity?

We microfabricated gold electrodes and coated them with SU8. However, when we bonded PDMS onto the SU8, the electrochemical activity of the electrodes dramatically decreased. Any ideas what could...

07 May 2020 3,727 0 View

Hi, Do you know of trail/path automated identification software?

I'm trying to identify animal paths in vegetation from drone created maps - using automated image analysis. Any suggestions?

06 August 2018 5,037 1 View

Dear fellows, i will be happy to receive articles for publication as the guest editor of disease markers journal IF 2.7, enclosed the details

Deadline is enclosed in the picture attached

30 June 2017 4,652 1 View

Rice sterilization process. Any leads to deal with nasty fungi on wild rice?

Hi, I am working with wild relatives of rice.I have been facing an issue with the germination as the seeds are highly contaminated. I tried to use ethanol 70% 2 minutes, bleach 4% for 30 minutes,...

07 November 2016 9,641 10 View

Anybody has experience with the New Brunswick Galaxy® 170 S CO2 incubator for cell culture? Is it any good?

Need some review/ reference on the product, is it a good buy?

27 December 2014 10,176 1 View

Does ECS Transactions has an impact factor?

I'm trying to find out if ECS Transactions (ECST), which is part of journals of the Electrochemical Society, has an impact factor, and if yes, then what it is.

08 September 2014 4,059 9 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

How to build my own lab made four point probe set up?

Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...

30 July 2024 906 2 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

Saleh Alkarim

Hello

visit these website will help you and read chapter 24 of MicroRNA Expression Detection Methods book .

Good Luck

http://www.molecular-beacons.org/MB_SC_design.html

http://link.springer.com/chapter/10.1007/978-3-642-04928-6_24#page-1

http://www.biosyn.com/tew/Design-rules-for-Molecular-Beacons.aspx

https://www.idtdna.com/pages/products/gene-expression/molecular-beacons

http://www.sigmaaldrich.com/technical-documents/articles/biology/molecular-beacons.html

Yoav Lubelsky

Thanks Saleh.

Victoria Diaz

Hi Yoav,

How did your probes turn out?