Hello

visit these website will help you and read chapter 24 of  MicroRNA Expression Detection Methods book . 

 Good Luck

http://www.molecular-beacons.org/MB_SC_design.html

http://link.springer.com/chapter/10.1007/978-3-642-04928-6_24#page-1

http://www.biosyn.com/tew/Design-rules-for-Molecular-Beacons.aspx

https://www.idtdna.com/pages/products/gene-expression/molecular-beacons

http://www.sigmaaldrich.com/technical-documents/articles/biology/molecular-beacons.html

Thanks Saleh.

Hi Yoav,

How did your probes turn out?