I want to design molecular beacon probe, anyone have suggestion regarding the best way to choose the sequence and the stem sequence?
Hello
visit these website will help you and read chapter 24 of MicroRNA Expression Detection Methods book .
Good Luck
http://www.molecular-beacons.org/MB_SC_design.html
http://link.springer.com/chapter/10.1007/978-3-642-04928-6_24#page-1
http://www.biosyn.com/tew/Design-rules-for-Molecular-Beacons.aspx
https://www.idtdna.com/pages/products/gene-expression/molecular-beacons
http://www.sigmaaldrich.com/technical-documents/articles/biology/molecular-beacons.html
Thanks Saleh.
Hi Yoav,
How did your probes turn out?
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