Please, I need a method to transfect MCF-7 cells with mCherry. I would like to maintain the mCherry expression in the cells for six months because I'm going to do a xenotransfection in mouse. What lentivirus have you used? Many thanks!
Joseph Papamatheakis
If you have the lentivirus -mCherry you must package fist in 293T and then infect MCF7. You can follow the process by fluorescence . There many lenti types, usually 2nd and 3rd generation ( no TAT requirement) . You must know what is yours to decide the type of packaging plasmids you need. .
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Ferenc A Scheeren
Hi
mcf7 is relatively easy to transduce with either lentivirus or retrovirus. You can use basically any vector. I use the lentiviral vector pcdh a lot and pBabe as a retroviral vector
good luck
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