How to find if Qualitative and Quantitative (discrete) variables have a relation?

I'm going to do a brief research about the dependance between the number of cases of Covid-19 and sex( male/female) . One of the requierements is to use inferential statistics to test the...

18 February 2021 7,092 11 View

Does anyone have a qPCR protocol for Lactuca Sativa?

I need to extract RNA, produce cDNA and do my measures via qPCR. How much material? Which stage of growth?

24 January 2021 559 2 View

Is PROCESS v3.5 by Andrew F. Hayes compatible with SPSS v 26 or 27 on Windows?

I installed the PROCESS Custom Dialog v3.5 by Andrew F. Hayes on two different versions of SPSS (26 and 27, both on Windows 10) running SPSS as an administrator and using the 'extension' menu....

30 November 2020 1,390 3 View

What are the digital competencies that university professors/trainers need to perform well & answer the demands of digital transformation businesses?

I would like to have access to academic research that compares the skills needed by university professors and corporate education instructors or facilitators. I would like to understand if the...

29 November 2020 442 3 View

Is there any research on in-vitro meat about sporadic cancerous cells and their possible impact?

The high rate of proliferation required of stem cells could produce genetic instability which may result in sporadic cancerous cells. Is there any research on this?

10 September 2020 3,666 1 View

Which is the best transfection method for cell lines that growth in suspension?

We are trying to transfect lymphoma cell lines, and we have tried several methods, the best is through the use of lentivirus of course, but in this pandemic days is very difficult to import this...

06 July 2020 8,043 12 View

Do you have in your country a regulation on sewage sludge in agriculture?

If in your country a regulation on sewage sludge for agriculture exists, please could me specify which microbiological parameters and limit values have been established? Thank you very much in advance

04 December 2019 8,690 3 View

Social media_systematic participation to giveaways?

I am analysing the outcomes of a pilot project involving people' reaction (reaches and engagements) to posts related to plastic pollution. To enhance engagement we proposed "challenges" i.e....

10 November 2019 571 0 View

Problems with RT-PCR of respiratory syncytial virus. Any advise?

Hello! I am having difficulty amplifying the coding sequence of RSV F protein. The protocol I have used is a sfollows: I infect HEp-2 cells with the virus, then when CPE is evident I collect the...

02 October 2019 3,061 3 View

Where does a single nucleotide ambiguity in sequencing of a digested plasmid, that was not in the original plasmid, come from?

We have been having issues with this certain plasmid for a while: we were trying to remove one gene (GFP) and replacing it with another. The digest of the original plasmid with BamHI and NotI...

11 August 2019 8,202 8 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

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03 March 2021 5,360 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View