What kind of lipids you need to investigate? Lipidomics?

Did you read what the lipid popes write:

http://lipidlibrary.aocs.org/topics/extract2/index.htm

http://www.cyberlipid.org/extract/extr0002.htm

http://www.lipidmaps.org/protocols/index.html

First like always grind the samples in liquid nitrogen. For long-chain-fats you need chloroform or dichlormethane definitely in the extraction liquid. chloroform 2:1 Methanol is still the best method to extract lipids completely. No other organic solvent can beat chloroform because it's breaking down the structure of the tissue. For MS dont use any plastik with chloroform. Work only with glasware, PTFE or metal. Then lipid fractionation with HPLC, TLC or SPE (Kaluzny) . For protein others may talk...

Thanks Alexander!  I will check the sites. The focus is indeed on a broad coverage of lipids. i.e. lipidomics. My own expertise is most on proteins.

For lipidomics you have to read Han. X, Gross and Murphy reviews.

This is a good state of art review:

http://www.ncbi.nlm.nih.gov/pubmed/21755525

And think about internal lipid standards (not existing in the sample) to add before extraction.

http://www.avantilipids.com/