I am planning to perform a global analysis of gene expression for the first time. I will be focusing on angiogenic factors. We are using mouse samples. Does anyone have any experience with such analysis? What are your tips and recommendations? PCR array, macroarray or microarray and from which company?
I personally was thinking about the RT2 Profiler PCR array for mouse angiogenesis (PAMM-024z; SABiosciences)... anyone with experiences with this array?
Hi Bart, I know some people will shake their heads now, but microarrays are somewhat obsolete by now. IMHO you should go with a more modern technique like RNAseq. If you are really only interested in angiogenic factors, I would go with a focused qPCR plate like e.g. https://www.lifetechnologies.com/order/genome-database/browse/plate/keyword/angiogenesis hope this helps, best, Andreas
Hi Bart, want to do it yourself? otherwise check the website of Exiqon. Speak you later, and good luck!
Bio-Rad's PrimePCR pathway plates are also a good alternative. They have several angiogenesis plates and each assay is fully wet-lab validated according to MIQE guidelines.
http://www.bio-rad.com/en-be/prime-pcr-assays/pathway/angiogenesis-collection-panel
Thank youall so much for your suggestions! I will check out all the options you suggested, and hopefully I will know more about the gene expression changes inmy knock-out mice soon!
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