Bart Krist

5 Questions 14 Answers 0 Followers

Questions related from Bart Krist

Does anyone has experience with succesful transfection of primary (murine) sattelite cells?

Dear all, I am trying to optimize siRNA transfection of primary sorted satellite cells from mice. Previously I had a lot of succes with transfecting murine myoblast cell lines with JetPrime...

06 June 2017 727 5 View

What can cause swelling in the healthy leg after hindlimb ischemia?

Hi everyone, I am operating mice to induce ischemia in the left hindlimb by ligating the femoral artery. The procedure is well established i our laboratory and after three years of working on the...

12 December 2015 4,904 7 View

Does anyone have experience with global analysis of gene expression, and which method is the best?

I am planning to perform a global analysis of gene expression for the first time. I will be focusing on angiogenic factors. We are using mouse samples. Does anyone have any experience with such...

06 June 2014 8,100 5 View

Does anyone have experience with Ottix Plus fixation?

In our lab we use Ottix Plus and Ottix shaper dehydration and deparrafination media, as more safe substitutes of Ethanol and Xylene. However, when I was fixing and staining some muscle samples, I...

05 May 2014 10,100 1 View

Which incubator is the best for organoid culture?

Hello everyone, We are currently in the proces of ordering a new cel cluture incubator for our brain organoid cel culture. Now I never really had any preference for a specific type of incubator,...

01 January 1970 3,310 4 View