Does anyone have experience with estrogen pellets in nude mice? - FAQS.TIPS

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How to name this rock?

Dear RG community members, I have found this sample in our rock collection used with bachelor students enrolled in Geology studies. I do not know where this rock comes from. I have made a thin...

17 June 2024 9,525 23 View

How did this rock form?

Dear RG members, I have produced a thin section of a rock in our rock collection, used for students enrolled in the second year of the bachelor of Geology at Sapienza University of Rome....

17 June 2024 689 3 View

What is the best buffer to resuspend proteins extracted from oil?

I hope everyone is well! I extracted proteins from sunflower and olive oils using acetone and then washing with n-hexane. I used RIPA buffer to resuspend because I plan to dot blot these samples....

02 June 2024 10,007 1 View

Crestomer on Plywood?

Normally unsaturated polyester or more in general radicalic bonding pastes doesn't adhere to marine plywood, due to the inhibition effect of formaldehyde and tannins. Crestomer 1152 of Scott...

22 April 2024 1,988 0 View

ICan you complete a 2 way anove if you are missing one level of samples after data collection?

Completed dzt collection but there was not enough samples to complete one level/box out of four.

14 March 2024 3,565 2 View

How can rotational stiffness be easily calculated for a steel connection?

I am currently working on modeling a steel structure with bolted connections, and I'm looking to incorporate the suitable rotational stiffness k. However, I've found that reference codes like EN...

25 January 2024 4,895 0 View

Brain cryosections cracking after permeabilization and drying?

I am doing immunofluorescence of mouse brain cryosections. I collect 25 um sections onto 1% gelatin coated coverslips, let them dry ~30 min, then peel off the OCT, rehdyrate the sections with 0.1...

14 December 2023 4,054 0 View

Friction at negative loads in viscoelastic materials?

dear friends I was playing with some theory of friction in viscoelastic solids, perhaps you can help me finding if there is any research on friction at negative loads (therefore under adhesive...

28 November 2023 5,133 1 View

Does exist researches on shoulder ultrasound for GP's?

Does exist researches on shoulder ultrasound for GP's?

24 November 2023 8,374 1 View

Why olivine is so commonly transformed in calcite?

Basaltic (or generically MgO-rich igneous rocks) have olivine phenocrysts commonly replaced by calcite, expecially if found in submarine environments. Of course this is not the only way to...

18 October 2023 1,428 1 View

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View