05 September 2019 3 9K Report

Hi all,

I'm using a single-cell electroporation approach to fluorescently label mitral cells in the AOB. I use 50 uM of Alexa488 but it does not seem to diffuse into the entire dendritic tree.

Does anyone have experience with that?

Is my Alexa concentration too low? Should I try a different Alexa dye or do I have to wait long enough for the fluophore to diffuse?

Thanks!

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