Hi all,
I'm using a single-cell electroporation approach to fluorescently label mitral cells in the AOB. I use 50 uM of Alexa488 but it does not seem to diffuse into the entire dendritic tree.
Does anyone have experience with that?
Is my Alexa concentration too low? Should I try a different Alexa dye or do I have to wait long enough for the fluophore to diffuse?
Thanks!