Do antibodies levels decline at the same rate in men and women?

11 December 2016 4,764 2 View

Does ultracentrifugation efficiently pellet influenza virus from allantoic fluid?

I count the virus particles in allantoic fluid, then pellet at 30 000rpm for 90 minutes. When I resuspend the pellet & count the particles I find that I only recover 15% of the virus particles....

04 May 2013 4,600 12 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View