Thanks a lot Dr. Qassim Kshash for your assistance, but also I need to the method by which can I assess ACh concentration. On the other hand how to prepare brain homogenate.

Kind regards

Cell Biolabs’ Acetylcholine Assay Kit is a simple colorimetric assay that measures

the amount of acetylcholine present in plasma or serum, tissue homogenates, or cell suspensions in a 96-well microtiter plate format.

Each kit provides sufficient reagents to perform up to 96assays, including blanks,

acetylcholine standards and samples. Sample acetylcholine concentrations a

re determined by comparison with a known acetylcholine standard.

The kit’s detection sensitivity limit is 0.75μM acetylcholine.

http://www.cellbiolabs.com/sites/default/files/STA-603-acetylcholine-choline-assay-kit.pdf

Thank you so much Dr. Shaik Ali

Kind regards

Thanks a lot Dr. Kshash

https://www.researchgate.net/

Dear, Dr.Jabbar

We thank you for the question in which the benefits and answers are beautiful and wonderful

Dear Dr. Kshash

your contribution, response and excellent comments are best than my inquiry

Kind regards

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Dear Dr. Azhar

Thank you for your support

Kind regards

http://www.cellbiolabs.com/sites/default/files/STA-603-acetylcholine-choline-assay-kit.pdf

Dear Dr.Jabbar

     I think good link by Dr.Hiba