Jacquie

We haven't done oxytocin before but we have experience of other hormones in urine.  An immunoassay technique should be fairly straightforward.  Costs would depend on the expected number of samples.  Do you have an idea of numbers?

Regards

Kate

Dear Jacquie,

We are just about to setup and measure Oxytocin in Saliva by sensitive immunoassay for a group at the Institute of Education -UCL. We are also very experienced at urinary hormone measurement (and direct mass spectrometry of urine and other biological samples). Do you want to talk and look at timing and sample numbers because if we can combine doing your samples at the same time costs are much lower.

PS also you will need to correct for dilution my expressing it against urinary creatinine levels. Have you got that covered or do you need that also?

Best wishes

Ray

Hello Kate

Thank you for your response, apologies if you saw an earlier half concocted message . I was using my phone and it didn't like it!

There will be a minimum of 300 samples and a maximum  of 540 

 Hello Ray

Thank you for your response. How do I contact you to discuss this further?

No trouble.  If you would like a quote, please contact me:

https://uk.linkedin.com/pub/kate-jones/a/189/b96

Hi Jacquie, I have done OT assay in Urine for trial a long time ago and my assay is working well for that, but we needed to extract OT, as for blood sample, to avoid any cross reaction with hormone. But...I am not sure that it could be scientifically relevant. OT is not secreted as another gland hormon with a tonical release. It is a neuro hormone secreted by episodes during short period (By example not during all a nursing duration in human that is your model) and very rapidly destroyed by Oxytocinase and other protease in blood. In consequence, OT urine level are very very limited in concentration and sometimes under the detection threshold. Additionaly, Corpus luteum is another OT source that produce very important concentrations of OT in a tonical manner. If and ovulation occurred in the very fisrt time after parturition, you can have very high OT level during the 28 days after LH surge that should totally overlap OT relased during nursing. Finnaly, If you want to follow OT release due to Mother/ypoung interaction, you can forgot because OT releases are lower in concentration that during nursing episodes. Only blood and sometimes LCR or microdialysis will be able to follow this activity..and it is not ethically possible to perform that in Human mother. Sorry to give you negative advice but it is to avoid a long work. If you need any precision please, do not hesitate to contact me via RG or my professional mail adress (Marnet(at)agrocampus-ouest.fr). Best regards

Hi Kate - sorry for delay MAP Diagnostics is in Welwyn Garden City North of London

my work email is [email protected]