Hi everyone,

I have been working on purifing Tn5 transposase for quite a long time. Basically following the protocol from http://genome.cshlp.org/content/24/12/2033.full. Occasionally I got one batch of transposase having activity, but after around 3 months, activity was not detectable any longer. Other than that batch, I got no more active protein. According to protein gel, the protein size is correct, roughly 53.3 kDa. Is there any tricky step that I missed? Any suggestion or help would be appreciated!

Best!