Biocompatibility testing based on cytotoxicity test ISO 10993.
Dear all, I have monthly nutrients data from WOA (in a separate NetCDF file) extracted for a specific region. I would like to convert these data directly into a bin file (one of the separate...
30 January 2021 9,241 2 View
How to design, reverse primer with ambiguous nucleotides?
03 January 2021 6,762 4 View
02 January 2021 987 4 View
I am keenly interested in the synthesis of heteroaromatic ionic salts for use as an organic template. I want to synthesize alkyl pyrrolium chloride or bromide through a simple addition reaction...
30 December 2020 2,778 3 View
Articles describing the use of carbon dots in agriculture have been increasing recently. Again, studies have found that carbon dots size and shape and some other features are effective. Therefore,...
30 December 2020 1,139 3 View
Modeling and simulation for launch vehicle Title of graduation project for a master's thesis in mechanical engineering, specializing in Control Search assistance required? Thank you
28 December 2020 9,000 1 View
Hi guys, I would love to know how I could go about solving the above question. I currently have 2 different species distribution models, one created with the current climate projection and the...
14 December 2020 864 8 View
My prefered area is in Waste management & BIM , where I have read about using 4D BIM & 5D in waste management , but I have not reached a significant research gap to work on it, so is...
03 December 2020 1,294 2 View
Hi there! currently i am working with spin column kit for DNA extraction I've put almost 600 uL of buffy coat. after a lot of washing, the RBC's remained stucking in filter? How could i get rid...
25 October 2020 8,712 4 View
Dear colleagues. I would like to design the floating active inductor shown in the figure below. Please, how can I measure the value of the inductance, parasitic series resistance, and the quality...
10 October 2020 9,488 1 View
02 March 2021 3,060 3 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
