Does anyone have experience in detection of total number of up/down regulated protein spots and fold change between the control and treatment?

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Rosie Morland

I'm not familiar with protein spot analysis but perhaps SAM could be useful?

http://en.wikipedia.org/wiki/Significance_analysis_of_microarrays

I use it to calculate fold change and significant from qRT-PCR array data, so theoretically I imagine it could be used to compare other similar data sets where fold change is being investigated...

(Apologies if this is irrelevant!)

Vivek A.

Sanower,

Yes i have done the PDQuest analysis of my 2D gel and it is not very tough. Which version of PDQuest you are using ? You can download the practical manual of the same from the website of BioRad . If you have any specific question then feel free to contact me.

vivek