Rosie Morland

9 Questions 37 Answers 0 Followers

Questions related from Rosie Morland

Can anyone help with making sense of Oligonucleotide Control Groups - Sense/Antisense/Mixed Based?

Hi. I am extracting data for a systematic review/meta-analysis and have come across a paper which uses oligonucleotides as an intervention - they have sense, anti-sense, mixed base, and vehicle...

04 August 2014 1,644 3 View

How should I compare left vs right sides of the brain across 3 test groups?

I am looking at cFos activation in the brain, and have a few areas of interest. I am also looking at left/right lateralisation in each area. I have 3 groups (naive, vehicle, test) with uneven...

01 July 2014 9,285 3 View

What cameras do people use when recording animal behaviour?

Has anyone used a GoPro or other cameras aimed at a non-technical market? We use a SANYO cctv-style camera capable of recording under low-light/infrared, but have been thinking of upgrading for...

04 February 2014 6,831 18 View

Does anyone have any thoughts on the accuracy and usefulness of IR beam break vs video analysis (e.g. using software such as ethovision)?

More specifically, what is the highest density of IR grid in use and could it be used to generate a 3D model of the animal which could then be used to look at more complex behaviours? I currently...

29 December 2013 8,484 2 View

Samples size calculation for an observational study involving naive animals

I'm currently developing a protocol for an observational study looking at changes in naive animals subjected to repeated behavioural testing - particularly looking at individual phenotype...

07 November 2013 1,073 3 View

In reporting of IHC results should one refer to density or count?

I'm doing some cFos immunohistochemistry, and wanted to check the general consensus on reporting the results. My predecessor worked out the count/area (microns), and scaled this up to count/mm2....

23 September 2013 10,060 3 View

Determination of estrus cycle from brain tissue?

Does anyone know if this is possible? I work with females, and made the decision to remain blinded to their estrus stage - I know this can affect behaviour, but equally as this is rarely measured...

03 September 2013 1,164 4 View

Effects of social isolation on health - does activity make a difference?

I seem to recall reading of studies (eg work by Berkman and others in Sweden) that showed people who avoid human contact have lower life expectancy. I was wondering whether this effect is...

29 August 2013 9,584 5 View

Rearing in rats: what does it mean?

I study open field behaviour in wistar rats, and one of the outcome measures I use is rearing (i.e. standing on rear limbs). I have noticed that in some instances, rearing can be the most...

29 July 2013 7,965 19 View