Does anyone have bacterial 16S primers for the V1-V2 region for Illumina MiSeq?

12 December 2013 1 995 Report

We are attempting to amplify bacterial DNA from human colonic biopsies. Our bacterial DNA is outnumbered 10:1 with human genomic DNA, and this has been a problem downstream for Illumina sequencing.

Sören Franzenburg

Have you checked out the protocol from the Earth Microbiom Project?

http://www.earthmicrobiome.org/emp-standard-protocols/16s/

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