Does anyone have Arcidine orange staining protocol for hydrogel seeded cells?

28 February 2021 1 9K Report

what would be the recommended protocol of acridine orange staining on hydrogel seeded cells by looking in Fluorescent microscopy. can someone have an idea about the concentration of a stock solution of AO, How much AO/PI to use, and what Quantity of cells to use for hydrogel?

He Ren

Hi Faiza,

To staining cells and observe by fluorescent microscope, we use Calcein AM/PI to distinguish live/dead cells. For cells in hydrogel, mix your dye with culture medium and staining by replace the medium.

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