Hi there,

I have been having some trouble getting dissociated Xenopus RGCs to grow on PAA gels. I leave them overnight in horse-serum+growth media and whilst they stick fine to the glass they just don't seem to grow on the gels. Has anyone been able to successfully and consistently grow dissociated neurons on gels and if so I would be really grateful if you could share your approach or some tips. 

Thanks so much for your help.