I am using xCELLigence to develop a growth profile for my SH-SY5Y cells. However, it seems that these cells may in fact migrate with the applied current. I tested this theory by seeding a 16 well e-plate with 20000, 10000, 5000 and 2500 cells. I monitored these cells daily and in the top half of the plate we see cells clustering to the top (right) half of the wells, and for the wells at the bottom half of the plate, its seems that cells cluster at the bottom half of the wells. This is consistent with the circuitry behind the plate. I'm going to try coating the wells with fibronectin (an adhesion protein). Can anybody provide any experience, insight, or advice for these cells and the xcelligence system?
P.S. The cells are passage 5. I tend to use cells between 7-18 passages, and split them at 70-80% confluence. The well size of a 16 well e-plate is similar to that of a 96-well e-plate, i.e. similar to an elisa plate.
Dear Justin,
Regarding your PS: With the new cell-based methods, which usually requires improved protocols for cell treatment thanks to there good sensitivity, it is indeed important to keep track of the passages and noticie any difference in behavior of the cells.
With respect to your xCELLigence system I can recommend you to check out the cell-based biosensor from Attana, www.attan.com.
Teodor
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