Does anyone have any experience using Circos graph?

More Nilson Antônio da Rocha Coimbra's questions See All

How to determine/find the "ideal design sample space" in the Doehlert Experimental Design?

When validating the Doehlert Matrix Design, do we encounter the issue of the "ideal sample design space"? I mean, in designs like the Central Composite Design (CCD), validation is usually...

21 September 2023 9,747 1 View

Can duckweeds change the pH of the medium that much?

I have been growing duckweeds in Clark solution (pH 6.5) and have noticed a 2 log pH shift down . I have checked the solution (blank) and there is no pH change in it. Does anyone know of an...

17 February 2022 3,840 2 View

Why won't BL21 Rosetta2 Colonies won't grow when transferred to liquid medium?

Hello, I purchased competent Rosetta2 cells (a BL21 expression strain of E. coli) and my transformations yielded 30-50+ CFUs per SOC plate. However, when I transfer the colonies to liquid culture,...

27 January 2022 8,953 0 View

How to compare two Spearman coefficients?

I have two significant Spearman coefficients from independent groups and I need to compare them. However, one negative (-0.421) and one positive (+0.601). When I apply Fischer's Transform Z test...

08 June 2021 1,766 4 View

Can you make glycerol stocks from super broth?

My PI tells me that you can't make glycerol stocks out of E. coli cultures grown in super broth. However, I can't find anything to substantiate his claim. Is this true? I've made glycerol stocks...

27 July 2020 4,331 2 View

How to convert Sentinel 2-L2A radiance into reflectance?

I downloaded a lot of images from https://scihub.copernicus.eu/dhus/#/home in the level 2A. When I open the images in QGIS, the bands have a value range in the order of 9000. This means that the...

26 June 2019 297 7 View

What are the risks of the "righ to the city" institucionalization (In the light of Henri Lefebvre)?

Currently, the right to the city is present in international treaties and even in the constitutions of some countries. However, the institutionalization of the right to the city is just the...

15 March 2017 4,948 6 View

Does anyone know of reliable antibodies I can use to label Glutamatergic and GABAergic neurons?

I'm looking for antibodies which count glutamate and GABAergic neurons in rat brain slices. The staining protocol I use is free-floating immunohistochemistry so if anyone has used antibodies for...

27 June 2016 6,014 2 View

Can anyone recommend cell lines that express cholinergic markers or are cholinergic neuron-like?

Hi, I am trying to find a cell line with neuron-like cells that are of the cholinergic type. Can someone advise me on that? Perhaps a cell line with no or less constraints when it comes to...

17 September 2014 6,713 1 View

Is the MnTiO3 synthesis possible by hydrothermal method?

Can anyone suggest the most appropriate step-by-step to prepare MnTiO3 nanoparticles by microwave-assisted hydrothermal method?

06 September 2014 3,575 3 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Radina Nikolic

Circos website ( http://circos.ca/) is very reach with resources and documentation

There is a link to forum where technical/software questions are discussed. For unique questions you can contact Martin.

Alena Zolotarenko

I have used Circos to visualize a table of transcription factors with their targets https://www.nature.com/articles/emm201697/figures/2