My PI tells me that you can't make glycerol stocks out of E. coli cultures grown in super broth. However, I can't find anything to substantiate his claim.

Is this true?

I've made glycerol stocks of E. coli with 2xYT before... which has about half the nutrient content of SuperBroth. So, I would assume super broth would be just fine to make a glycerol stock with.

Thanks

More Daniel Nilson's questions See All
Similar questions and discussions