My PI tells me that you can't make glycerol stocks out of E. coli cultures grown in super broth. However, I can't find anything to substantiate his claim.
Is this true?
I've made glycerol stocks of E. coli with 2xYT before... which has about half the nutrient content of SuperBroth. So, I would assume super broth would be just fine to make a glycerol stock with.
Thanks