I am investigating the feasibility of validating my IHC-based protein expression analysis in FFPET samples using TaqMan protein assays. I wonder if anyone has any experience with these assays?
I am interested in their accuracy when using protein extracted from FFPET, the ease of creating protein-specific probes with user-supplied antibodies and the difficulty in identifying suitable controls?
Shadi Khadijeh Gholami
Rachel, I have never used that, but supplier claim that is more accurate than western blot in term of quantification, however its accuracy is 75%. This is what they said almost 2 years ago, I am not sure if it was any improvement.
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Ratan Choudhary
I was also thinking about it. Sharing experience would be greatly helpful for beginner.
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