I just want to do a heat kill on a faecal sample to ensure that all bacteria are dead. I have tried 100oC for 30 minutes and still see live bugs.
Doubt you got penetration throughout the material. USDA indicates an internal temperature of 160F will kill enteric bacteria in meat. Time is not considered a factor. https://www.fsis.usda.gov/wps/portal/fsis/topics/food-safety-education/get-answers/food-safety-fact-sheets/meat-preparation/ground-beef-and-food-safety/CT_Index
Hi
fecal sample from human?
what did you means about live bug?
in my knowledge there is no enteric bacteria of human can tolerate 100 degree except spore of clostridium.
please explain more about your method.
good luck
If only enteric bacteria is you target for killing, 80 oC for 15 minutes is sufficient. It seems that you have tried higher temperature for longer period of time and failed to do so. I think the growth after the heat treatment do not represent Enteric pathogens. It represent Spore forming bacteria.
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