How can I detect both high and low molecular weight proteins on the same blot?

I am running some western blots currently and need to detect 2 proteins with relatively different molecular weight. one is 63KDa and the other is 262KDa. I am looking for ways to modify my...

27 October 2016 9,492 5 View

What are the roles of teachers towards education for sustainable development?

What roles should teachers play in their locality to promote sustainable development?

29 April 2014 9,087 9 View

Which statistical package or software application is easiest to use by non-statisticians?

Can you recommend a simple-to-use statistical software for a non-statistician?

05 March 2014 8,656 44 View

Why are many roads (mostly flexible pavement) in the most developed nations having pavement distresses?

Why are many roads (mostly flexible pavement) in most developing nations having potholes, cracks, ruts, depression, etc.? What are the economic implications of this? Are suitable construction...

17 February 2014 7,375 9 View

Kindly comment on the agrose gel electrophoresis image attached?

Dear Colleagues, I isolated DNA from the liver of rats treated with an environmental contaminant and those treated with both the contaminant and different vitamins. I later ran the DNA isolated...

01 January 1970 5,821 8 View

Do the introduced salts (NaOH/ HCl) influence the spectroscopic shifts of the fluorescence excitation-emission matrix?

During the resin (XAD-8) fractionation of wastewater salts (NaOH or HaCl) are used for acid or base elutions/pH adjustments. Since the amount of introduced salt is minimal, so is it required to...

08 February 2021 9,489 2 View

How to find degree of deacetylation of chitosan colorimetrically?

I am working with low DD% chitosan. It doesn't dissolve completely in acidic solutions. How to calculate the degree of deacetylation by potentiometric and acid-base titration, UV spectroscopic...

27 January 2021 8,532 2 View

Looking for high efficient light sources (Laser, HE LED) for Spectroscopic Ellipsometry?

Conventional light sources have a penetration depth of a few nanometers in metal layers and cannot be used for spectroscopic ellipsometry purpose to measure layer thicknesses of a few 100 nanometres.

27 October 2020 7,663 4 View

Did anyone try to measure the thickness of films with fluorescent peptides immobilized by ellipsometry?

I immobilize fluorescent peptide on chitosan films and when I try to measure the thickness of the film with ellipsometry, the software says that I have a film with 2 nm or less. However, with...

01 October 2020 3,702 3 View

What is the possible reason behind the unexpected behavior of 4cp-ferrocene during its characterization using a spectroscopic technique ?

Often, any ferrocene derivative during its characterization using a spectroscopic method gives two absorbances, along the ultraviolet region and the visible region (figure 1 ). in vitro was...

05 May 2020 4,652 6 View

How the dose rate was experimentally determined, and how this dose rate can be applied to a system such as Cr2O3. thin films ?

07 August 2019 3,875 2 View

How we can investigate the resistance of AlN thin films is good enough to get a high piezo property material?

Hello, I would like to ask the possibility of failure piezo properties performance of AlN thin film deposited by reactive sputtering? I have good ellipsometry, XRD & SEM results but poor...

20 June 2019 7,930 4 View

How we can investigate the resistance of AlN thin films is good enough to get a high piezo property material?

Hello, I would like to ask the possibility of failure piezo properties performance of AlN thin film deposited by reactive sputtering? I have good ellipsometry, XRD & SEM results but poor...

05 June 2019 8,955 5 View

What is the best software for Ellipsometry measurements?

I have been checking different software packages in order to find the most suitable one based on my work. Considering some parameters as the basic steps of measurements, modelling/ data input,...

10 November 2018 9,421 0 View

Can I trust spectroscopic ellipsometry measurements of a nanometer-scale thin film?

We are fairly new to spectroscopic ellipsometry (SE) analysis and we want to be sure we are not utilizing this technique outside its capable range We have amorphous carbon thin films...

26 August 2018 4,632 3 View