Coul you please share which type of study you were conducted on the animals, what was the detailed protocol? Which local drink was used to feed the animals for 42 days?

It is  a local  Nigeria drink  that is widely consumed. I tested the effect  of chronic   consumption of the drink in rats. The animals were given the drink orally for 42 days. They were then sacrificed and I consistently observed the gross lesion in the liver. I  will soon published the detailed result. I only need  pathologist to tell me what the lesion are

 you have several cysts in the liver of the first picture and lesser number of the same cyst while the last picture has no cysts at all only the gallbladder.

to know the exact pathological lesion a histological section is required.

i expect that there is a tabe worm investation in your rat colony. 

Taenia taniaeformis

http://dailyparasite.blogspot.com.eg/2010/05/may-25-taenia-taniaeformis.html

Tapeworm cysts were identified in liver of Wistar rats and it induced fibrosarcoma in liver

https://www.researchgate.net/publication/223211835_Strobilocercus_fasciolaris_infection_with_hepatic_sarcoma_and_gastroenteropathy_in_a_Wistar_colony

Echinococcus multilocularis

http://theconversation.com/the-top-ten-parasites-that-could-be-lurking-in-your-food-29015

or certain type of protozoa.

Article Strobilocercus fasciolaris infection with hepatic sarcoma an...

Thank you Hosam.  Are there other causes of the cysts?  The animals  were fed with an alcoholic (20%) based herbal drinks  that is widely consumed in Nigeria. Moreover, I treated the animals with  an antibotic and anthelmintic  before the commencement of the study.   In addition, the cysts were not found in the control. More importantly, I have done the histopathology analysis and noticed severe lesion (necrosis) in the liver of treated animals

 For the attention of Amit

The animals were fed with an alcoholic (20%) based herbal drinks that is widely consumed in Nigeria

Necrosis is probably from the high alcohol content for such a long duration.  I would stain for fibrosis because, you have probably induced cirrhosis and that is hepatocellular carcinoma.  Just a guess but that's what it looks like.  You would need to post some H&E sections and have a histopathologist look to be sure.  

Thank you Kelly,

I have some of histopathological slides the revealed severe necrosis, vacuolation, sinusoidal congestion and peripheral fibrosis, edema and  atropy of the cords

yes it is better to get histopathological sections if these lesions are hepatocellular carcenoma. then the alcoholic drinc is very carcenogenic. usually you get fibrosis so the histopathological section and liver enzymes will help.

Some histopathological  images and section. Kindlly interpret it for me

Your histology fields need to include the cyst. Having said that, looking at your macroscopic fields, this 99% looks like a liver cyst, from a parasite, and does not look characteristic of HCC. Don't simply assume that your antihelminthic dose would have eradicated this. They have different specificities. For example, ivermectin is not effective for tapeworm.

Ok. Thank you

I think if this is wild rat.. this multicystes is the larval stage of Taenia fasciolaris or Cysticercus fasciolaris, if this is lab rat also the same result..Why??????

Because your lab. house did not close strongly and their was a contact between lab. and wild rats...

you can be sure by cut one cyst cearfuly and examine under microscope and can see the scolex