I am trying to isolate cardiomyocyte cells without using the langendorff. If you have any suggestions or a method you have used that has worked that would be greatly appreciated if you could share that with me.
Thank you for your time!
Hi Emiri,
Take a look at this link. I previously attempted to isolate adult mice cardiomyocytes without lagendorff but i wasn't successful. I hope this link will help.
Good luck.
https://www.researchgate.net/post/Can_any_one_suggest_me_to_find_a_way_to_dissosiate_adult_cardiomyocytes_without_using_langendroff_appratus2
You may try the protocols used for bigger (canine, human) hearts (e.g, Voigt et al, 2013), but, believe me, using coronary perfusion gives much better results.
I have also heard of mechanical dissociation (McDonald et al, 1998) may work, but I never tried this one.
Both links are below.
Good luck
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2231141/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3731177/
Thank you so much for your help; definitely interesting and will look into it more!
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