How do I interpret fit statistics SEM and Modindices output?

Hi, I would need some help in understanding the SEM output. I run a latent change score analysis to test the change score of a variable of interest at two-time points (repeated measure) PLD1 and...

05 January 2021 3,367 2 View

Lipofectamine 3000 in 10 cm dish?

Dear all, I'm trying to transfect HeLa cells with lipofectamine 3000 in a 10 cm dish. Do someone of you have a protocol to share? I still examined the table for scaling up or down lipo3000 but it...

13 October 2020 2,926 3 View

Which is the best system to keep endocrinology samples frozen in the field, without electricity?

Hi everyone, I am looking for suggestions to store biological samples in the field for endocrinology analyses, where I will not have electricity. One of the options we are evaluating is a solar...

01 September 2020 3,766 3 View

How to combine beam and shell elements in ANSYS APDL?

Hi everyone! I have a doubt and I hope there is someone who can give me an advice. I am modeling a MEMS gyroscope for my Master thesis and I am starting with a very basic structural design. My...

20 August 2020 9,524 5 View

How to edit density and heat capacity in Aspen Plus ?

Recently I'm trying to edit a pure component mass density and heat capacity of an existing component NaNO3 but failed. Could anyone help me on this? Thanks a lot.

17 July 2020 8,800 8 View

Do you have suggestions about CROSS CULTURAL ADAPTATION of questionnaires?

Hi! I am currently translating and adaptating a questionnarire about attitudes towards inclusion, and I would like to have suggestions about specific literature in translation and cross-cultural...

23 March 2020 2,630 3 View

Can the pcDNA5FRT/TO vector be used for transient overexpression of a gene in a common cell line?

01 March 2020 3,206 2 View

Does CXCL8 or other chemokines induce in vitro cell proliferation?

hi everybody, i have some troubles with chemokines and cell proliferation. Different papers evidence that CXCL8 (but also CXCL12, CCL2 and other chemokines) are able to promote cancer cell...

03 February 2020 214 4 View

Anyone could suggest how to analyze calcium imaging using imagej?

Hi, we would like to analyze time sequences images of calcium imaging assay performed with Fluo4-AM in imagej. Is there any plugin that we could install to do this? Do you have any kind of...

27 January 2020 2,665 1 View

Chloroform and isopropanol RNA extractions?

I have to perform RNA extraction with TRIzol LS and I just realized that we have chloroform stabilized with ethanol. Is that ok? And should chloroform and isopropanol be "molecular biology...

30 September 2019 4,647 5 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What low-cut filter (high Pass frequency) to be used for response spectrum (Tripartite plots) ?

What low-cut filter (high Pass frequency) to be used for the response spectrum (Tripartite plots)? I am currently working on differentiating between hard/soft site responses in Indo-Gangetic...

21 February 2021 9,273 2 View

Absorption behaviour of anechoic model?

I have modelled a unit-cell of an anechoic structure using ANSYS. The layer is silicone and has been assigned as an acoustics physics region, with an air cavity inside (the air has been modelled...

18 February 2021 4,119 1 View

FLIM Signal to noise ratio of a time series - can I use dark frames to measure noise?

I am doing an analysis on some FLIM data in the form of time series image stacks. I need their signal-to-noise ratio. Recording starts several picoseconds before the excitation pulse, so no sample...

08 February 2021 7,169 3 View

How to eliminate noise when using a sensor with instrumentation amplifier and giving it's output to Arduino?

When I am trying to implement a pressure sensor through instrumentation amplifier and then giving it's output to Arduino, I am getting garbage value due to noise, so how to eliminate it.

05 February 2021 8,598 11 View

Need a research paper for multi speaker and single microphone acoustic echo cancellation?

Hi, I am working on a problem that involves multiple smartphones/tablets. All devices are playing the same sound with synchronization difference up to +- 5ms. Now, there is an echo issue and I...

02 February 2021 5,929 3 View

How can I quantify background noise in a bioacoustic file?

I am using Audacity to analyse the presence of birds and frogs recorded with a SM4 recorder (Wildlife Acoustic). Background noise (rainfall, wind) is an important variable to consider that can...

28 January 2021 2,989 3 View

Where can I get a Music therapy database for Alzheimer's Disease?

I am working on the effect of music therapy on persons with Alzheimer's Disease. I require EEG signals of healthy individuals and people with Alzheimer's Disease/Dementia that have undergone music...

22 January 2021 8,613 5 View

Why doesn't semen analysis usually use darkfield microscopy?

From examples I've seen online, brightfield has too-low contrast and phase contrast has large (diffraction?) halos that can look like noise.

15 January 2021 3,155 1 View

What is the formula used for the calculation of L10?

Is there any formula, which can be used for the calculation of L10 i.e, peak noise level? Please give your opinion?

14 January 2021 8,408 3 View