Can anyone recommend a good mouse transcriptome database that is organized based on tissue?

Can anyone recommend a good mouse transcriptome database that is organized based on tissue? I am looking to find a list of protein coding mRNAs detected in whole blood.

28 August 2020 1,160 2 View

How can I transmit on ELF and VLF with a small form factor?

I am looking to transmit ELF through VLF using either just a +20dB signal generator or less than 10W amplifier over a distance of no more than 2 miles from a very small form factor. In the past,...

04 March 2020 2,818 5 View

Do you need salt in column buffer for chitin bead purification?

I would like to purify a protein using the IMACT system where my protein has an intein and chitin-binding domain. I cannot use salt in my purification. The protocol says that you can use 50-1000...

28 August 2018 7,691 3 View

MBP fusion protein not binding to amylose beads?

I have a MBP fusion protein that is not binding to amylose beads. I have already tried adding glucose to the media (although I did not add it to my starter culture) and I am not using detergents...

22 August 2018 5,726 7 View

How can I randomly assign participants in intact groups?

I doing a true experimental study in which I am examining learning behaviours among students. How can I give half of the class the treatment, and the comparison group an alternate activity without...

23 April 2018 5,348 4 View

How can I randomly assign participants in intact groups?

I doing a true experimental study in which I am examining learning behaviours among students. How can I give half of the class the treatment, and the comparison group an alternate activity without...

03 April 2018 1,886 5 View

Why am I obtaining non-specific bands for tissue samples?

I am using western blot to investigate the level of expression of the g protein, Gq, in cardiovascular, renal, and arterial tissues. I have been getting prominent bands at 42 kDa and 25 kDa. The...

18 December 2017 5,976 2 View

Does anyone have a good link for insulin purchase for MCF7 cells?

Looking for a good source to purchase insulin for cell culture!

28 August 2017 7,056 1 View

His-tagged protein not binding to nickel beads, any help?

I am trying to purify a fusion protein using the E.Coli expression system. I have successfully expressed my protein and verified this using an anti-His antibody and an antibody that recognises my...

04 February 2017 4,336 5 View

Does anyone have an explanation with what happened during this BET run?

I have modified USY with KOH, which upon XRD seemed to destroy the framework structure anyway. I ran an BET of the sample using N2 at 77K and the result does not show any form of isotherm really...

08 April 2015 8,277 9 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

How to compute the internal consistency of a scale with ordinal items?

Hi community, I am trying to determine what is the best way to calculate the internal consistency of a newly developed self-esteem scale. I am working together with two other researchers on the...

28 February 2021 1,369 5 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View