dear all
I work on CBA/J (F) x DBA/2J (M) mice as abortion models. Since I received them, I have added walnuts, multivitamins, carrots, and apples to their diet in a limited way and maintained them in an animal facility, but so far I have not been able to see vaginal plugs in them. Does anyone have a suggestion for getting pregnant in this model?
You need to wait and check the vaginal plugs twice a day, and you also need to check the mouse week age. Since the CBA/J mouse is easily frightened, time is needed to make them to familiar with each other. So you just put them in the same animal facility on consecutive days and you will see it.
Zhengjuan Li
All this has been done however the vaginal plug is not seen.
Hossein Ansariniya Then, I don't know the exact reasons. Maybe, you can change the female mouse or male mouse. Maybe, your mouse really doesn't want to mate.
By the way, I wonder if you are inducing the spontaneous abortion model. Recently, I failed to do it using the CBA/J X DBA/2J mouse model, and I can't seek the reason. Therefore, I hope you can share your mouse purchase information if you can induce the abortion in the mouse model. I will be grateful for your share.
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