Hossein Ansariniya

6 Questions 7 Answers 0 Followers

Questions related from Hossein Ansariniya

Reduced and non-reduced protein speed in SDS-PAGE?

Why is the reduced protein (with 2-ME) placed higher than the non-reduced one in SDS-PAGE?

18 October 2023 1,659 1 View

Why products more than 200 base pairs are not amplified in real-time PCR?

Why products with more than 200 base pairs are not amplified in real-time PCR? Are there any images of this?

31 January 2023 2,029 3 View

What is the best formulation for recombinant protein stability during freezing?

I have liquid recombinant protein. What is the best formulation for recombinant protein stability during freezing?

11 December 2021 8,449 6 View

Does anyone have a suggestion for getting pregnant in CBA/J (F) x DBA/2J (M) mice model?

dear all I work on CBA/J (F) x DBA/2J (M) mice as abortion models. Since I received them, I have added walnuts, multivitamins, carrots, and apples to their diet in a limited way and maintained...

16 November 2021 4,112 3 View

How can I prevent recombinant protein aggregation before, during, and after dialysis?

I am purifying a 20 kDa recombinant protein. Protein concentration is greatly reduced after dialysis. My dialysis buffer is 20 mM Tris buffer pH 8.0 that contains 150 mM NaCl, 10% glycerol. How...

15 September 2021 7,884 8 View

How can I prevent the recombinant protein from aggregation during dialysis?

I am purifying a 20 kDa recombinant protein. Protein concentration is greatly reduced after dialysis. My dialysis buffer is 20 mM Tris buffer pH 8.0 that contains 150 mM NaCl, 10% glycerol. How...

21 June 2021 1,830 5 View