Hello everyone, we are starting to work with lignin peroxidase from fungi, and we have a question regarding the assay because the extracts often already have a significant color. However, what matters is the increase in absorbance at 310 nm due to the release of the product after adding H2O2. This would mean setting the absorbance to zero at 310 nm before adding the hydrogen peroxide, correct? Does anyone have a reliable protocol with a reference? Thank you in advance for your help.