PCR water or molecular grade water for RNA samples?

Hello everyone, It's my first time working with RNA but I am told it's very easily degraded. A protocol for cDNA synthesis requires "nuclease free water". Should I use PCR water or molecular grade...

27 May 2020 9,480 11 View

How to plot eye tracking data on video?

We collected eye tracking data as participants went through an hour's worth of gameplay for which we have video captures. How would I go about plotting the raw eye data on these videos so I can...

26 February 2020 1,585 2 View

Biosafety cabinet for RNA extractions necessary?

I am extracting RNA from milled plant material (BSL1) for downstream RNAseq. If possible, it would be great not to take over the only biosafety cabinet in the lab for RNase free space for weeks...

11 September 2019 8,712 4 View

Has any reader of this question, access to such neural network or other ML tool that predicts the next symbol given some prior sequence?

I am researching random number generation techniques, and would like to find a neural network or other machine learning software, that is able to track the sequences that my software generates, in...

17 February 2019 9,564 2 View

What is the most appropriate method for sampling gas from a closed system?

I would like to measure gas production in a small closed system, i.e. 40 ml tube with PTFE septum. I am wondering if it is more appropriate to continually sample from one tube over time and simply...

12 December 2018 8,441 3 View

In what ways does genome architecture reflect cell architecture?

In simple self-replicating machines (exclusive of developmental automata), the structure of the description (machine genome) is very simple. For the von Neumann model, the description is...

10 October 2018 6,387 3 View

Supervised classification in R?

I would like to perform supervised classifications in R on a large dataset of photographs to assess land cover change. Has anyone attempted this for landscape photographs in R? And is there value...

24 May 2018 4,522 4 View

We found a Cronbach's Alpha above 0.60 in 4 dimensions of our Questionnaire. The others 4were below 0.60. What should we do to avoid disrupting the Q?

We are building an almost "standardized" questionnaire according to Ellen G. White on the 8 natural remedies. When doing the factorial analysis, we found 4 dimensions with Alpha of Cronbach above...

17 May 2018 8,024 5 View

ER/PR and HER2 status of the EMT6 cell line?

Does anyone know the ER/PR and HER2 status of the EMT6 mouse mammary cell line? I think looking on line it is HER2 neg (not amplified) but I can't find a definitive answer. Thanks!

25 September 2016 4,082 1 View

No title

I need some articles that can help me understand electromagnetism in different animals.

04 September 2016 2,921 3 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View

How can I calculate teh crystallinity degree of graphene oxide?

I had treat my XRD diffraction pattern by Highscore to calculate the crystallinity degree Xc and I have the formula but I dont no the 2Theta of Ac and Aa to calculate Xc. Is it like polymers...

10 February 2021 6,305 5 View

Does Abaqus plugin AM-Modeler predict the same temperature/crystallinity history as UMATHT user-subroutine?

During AM process, progressively activation of elements can predict the temperature/crystallinity history, including surroundings heat losses and than this data could be used to determine the...

06 February 2021 4,686 1 View

Xrd result of ZnO thin film shown better crystallinity at low temperature and the crystallinity decreases as the temperature increased. why?

I have synthesized ZnO thin films at four different substrate temperature say, 300, 350, 400 and 450C. and the samples were annealed at 450C for half an hour. the xrd result shown better...

04 February 2021 7,641 4 View

What is the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow endothelial cells?

Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...

28 January 2021 8,765 4 View

How to gate mouse MDSC cells through flow cytometry?

On inducing the mouse bone marrow cells with X compound, there is a change in cells morphology and characteristics. In that case how to gate these cells, as these cells are showing suppressive...

27 January 2021 608 2 View

How much of cells needed to get good number of RNA ?

Hello I am growing MSCs cells in either 24 wells or 12 wells plate and then I need to lysate these cells for RNA extraction I ususally get RNA from 5 to 30s ng/ul Is there is any idea or tips...

26 January 2021 8,531 3 View

How to do rat bone Immunofluorescence, Please suggest?

Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo

26 January 2021 4,165 3 View

Hello, my name is siti. My question is what is the related issue in bone marrow transplant or hematology that I can choose for my research project ?

I am doing a master in nursing but still does not have any idea about what topic I am going to choose. pls help

25 January 2021 1,097 12 View