We need to use biofilms in cell culture experiments, where they will be compared with monoculture stimulation. Our focus is not the inter-species interactions within the biofilm, but the immune response of epithelial cells to it. Ideally I am looking for a protocol using the endotracheal tubes.
Thank you.
please review the following
Methods Enzymol. 1999;310:534-51.
Recovery and characterization of biofilm bacteria associated with medical devices.
Mittelman MW1.
www.fda.gov/.../MedicalDevices/.../UCM...
Biofilm Formation: A Clinically Relevant Microbiological Process
Rodney M. Donlan
+ Author Affiliations
Biofilm Laboratory, Centers for Disease Control and Prevention, Atlanta
Reprints or correspondence: Dr. Rodney M. Donlan, CDC Mail Stop C-16, 1600 Clifton Rd. NE, Atlanta, GA 30333 ([email protected]).
Abstract
Microorganisms universally attach to surfaces and produce extracellular polysaccharides, resulting in the formation of a biofilm. Biofilms pose a serious problem for public health because of the increased resistance of biofilm-associated organisms to antimicrobial agents and the potential for these organisms to cause infections in patients with indwelling medical devices. An appreciation of the role of biofilms in infection should enhance the clinical decision-making process.
Thank you for your quick responses. I am currently considering a protocol involving the cutting out of 1x1 cm of the tube following the biofilm detachment using vortex with zirconium beads.
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