Dear Natalya Torgasheva,

unfortunately you haven’t told us in detail

a) which dye you prefer (ThF-S or ThF-T) and

b) if you need the information for real staining (histological, Immuno-Fluor, EM?) or c) only for theoretical background and considerations.

In my files besides other references/articles I found the following, that my be of interest to you:

Mechanism of thioflavin T binding to amyloid fibrils, KHURANA R et al. Journal of Structural Biology 151 (2005) 229–238

Identification of the Core Structure of Lysozyme Amyloid Fibrils by Proteolysis, FRARE E et al, J. Mol. Biol. (2006) 361, 551–561 (incl.  TEM)

Binding mode of Thioflavin T in insulin amyloid fibrils, GROENNING M et al, Journal of Structural Biology 159 (2007) 483–497

Binding Modes of Thioflavin-T to the Single-Layer β-Sheet of the Peptide Self-Assembly Mimics, WU Chun et al J. Mol. Biol. (2009) 394, 627–633  (see also: http://www.sciencedirect.com/science/article/pii/S0022283609011954)

BANCROFT JD & GAMBLE M(Eds), Theory&Practice of Histological Techniques, a) pp.274-275; b)p-389 (6th Ed.Churchill&Livingstone,2011):

a) Meth forAmyloid-FLUOR-(e.g.vasculature)Micr.acc.to VASSAR&CULLING(1959) (Thioflavin T)

b)Meth for plaques & tangles (Alzh.Dis., brain) (Thioflavin S).

If you would like to have a look into one of these articles, let me know…(write to my e-mail at work: [email protected]).

Best regards and good luck

Wolfgang