Hi,

would anyone have a good protocol for denaturing IP from S. cerevisiae? I want to look at the phosphorylation state of a protein and therefore lyse the cells under harsh conditions that deactivate phosphatases. All protocols I tried so far didn`t work particularly well.

My preference would be to use GFP-tag, but I can also use others if necessary.

Thank you!

More Michael Plank's questions See All
Similar questions and discussions