Does anyone have a protocol for click chemistry on fixed (PFA 4%) brain tissue, preferably not with a click-IT kit. ?

More Cristián Gutiérrez-Ibáñez's questions See All

Can someone help with the instrument configuration in Profex using Rietveld Refinement?

I am trying to configure the instrument in Profex but I can't get a chi-squared value below 9.65 for LaB6. This is the configuration of the equipment, which is a Bruker D8 Advance with a Linxeye...

14 July 2024 4,767 1 View

Hi once i have requested a full text whats next?

I requested the full text of the book “Landscapes Ways of Imagining the World” de John R. Stilgoe, I am new in this so i dont know what to do next or how will i obtain the permission

23 June 2024 1,855 1 View

Which preprint platforms or servers are your preferred choice for Health Sciences?

I would like to know other researchers opinion about their preferred preprint servers/platforms

28 May 2024 8,291 0 View

PVP can be dissolve in 1- pentanol or 1- butanol? It's the same result?

at the preparation of the C18 alkyl silica, the paper used n-pentanol to dissolve PVP, in my lab I have 1- butanol. Could it be the same?

20 May 2024 5,933 1 View

¿En sus paises existe una evaluación a gran escala de candidatos al egreso de nivel licenciatura/pregrado/college??

En México existe una institución externa a la universidades que evalua a los candidatos al egreso, es decir, a los alumnos que se van a graduar de licenciatura. Es una prueba a gran escala, que...

08 May 2024 6,666 1 View

Where to find a simulation for propellers in ANSYS CFX or Fluent?

Where to find a simulation for propellers in ANSYS CFX or Fluent? See what I already done below:

01 May 2024 7,255 3 View

ANSYS FLUENT - I need to generate variable wind speeds with rapid changes in velocity?

ANSYS FLUENT - I need to generate variable wind speeds with rapid changes in velocity. How to add winds in ANSYS to simulate a hurricane? Or how to get a variable wind speed and direction input?

01 May 2024 4,236 1 View

How to simulate water in ports? Ansys fluent or CFX?

I need a tutorial for a simulation for water entraped in ports with various depth and various currents at the port entry. Where I can find a similar simulation?

01 May 2024 3,523 2 View

Distance or interaction constrained molecular docking tools?

Hi everyone, I'm looking for some open source software for molecular docking with constraints based on distances or interactions. Any recommendations would be greatly appreciated. Thanks!

16 April 2024 1,238 2 View

Science and teaching: Are the researchers better professors?

Most of the researchers use to teach at university. In some careers, professionals who exert their profession without doing research share teaching spaces. When I was a chemistry student, 100% of...

04 April 2024 4,483 6 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Does continues partial charging/discharging do any harm to the BESS performance and degradation?

For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...

01 August 2024 9,903 7 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

What is the best method to detect the chemical composition of pollen ?

I have multiple small samples of grass pollen with anthers. I want to find a method to identify concentrations of proteins, lipids, and other various elements for these samples. any suggestions?

28 July 2024 5,331 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View