Does anyone have a protocol for Brassica juncea transformation?

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How to calculate the mapping efficiency (% of uniquely mapped reads) for an aligner?

Hello everyone, I am working with Arabidopsis RNA-seq data and have used HISAT, TOPHAT and STAR aligners that gives a summary statistics showing the percentage of uniquely mapped reads and...

05 June 2018 6,491 5 View

How to find the insertion position of my gene of interest from genome sequencing of transgenic plants?

Hello everyone, I introduced a gene tagged with GFP to Arabidopsis, its expressing at both transcript and protein level and is showing the phenotype somewhat similar to what I expected. Now i...

07 August 2016 4,314 2 View

What is the minimum size requirement for LAMP-PCR amplification?

I want to amplify a short RNA sequence (about 170bases) but am unable to design LAMP primers as the software (Primer Explorer) gives the message that the minimun size is about 200 bases. Has any...

05 June 2015 4,911 10 View

Software for identification of alternative splicing isoforms from the data of RNA seq?

I am analyzing RNA seq data for the alternative splicing isoforms of my gene of interest, but unable to find a reliable and user friendly software/tool. Has any one (related to bioinformatics,...

05 June 2015 10,027 4 View

Has any one used the Oxford Nanopore's MinION sequencer?

Recently i came to know about a mobile DNA sequencer launched by Oxford (MinION). Has any used it? what about Its efficiency and the reagents required for sequencing?

05 June 2015 1,596 3 View

How can I confirm Agrobacterium (GV3101) transformation?

I am facing problems in confirmation of transformed GV3103. Transformed bacteria is able to grow on antibiotic selections media (Rif+Gent+Kan) while the wild type was unable to grow. But colony...

03 April 2014 4,194 15 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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01 August 2024 2,079 0 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

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How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

Massakib Bekkaye

Take a look on those papers

http://www.pakbs.org/pjbot/PDFs/45(3)/37.pdf

http://nopr.niscair.res.in/bitstream/123456789/5834/1/IJBT%203(1)%2097-102.pdf

Farhana Nazneen Chowdhury

Select first full sequence of gene of interest→ Gene cloning→ designing the gene→ cutting the gene apart with enzymes→ (Plasmid) bacterial transformation through E. coli.→ Co-culture of Agrobacterium with canola tissue→ Another experiment is done without plasmid for comparison.