How to calculate the mapping efficiency (% of uniquely mapped reads) for an aligner?

Hello everyone, I am working with Arabidopsis RNA-seq data and have used HISAT, TOPHAT and STAR aligners that gives a summary statistics showing the percentage of uniquely mapped reads and...

05 June 2018 6,353 5 View

How to find the insertion position of my gene of interest from genome sequencing of transgenic plants?

Hello everyone, I introduced a gene tagged with GFP to Arabidopsis, its expressing at both transcript and protein level and is showing the phenotype somewhat similar to what I expected. Now i...

07 August 2016 4,246 2 View

Software for identification of alternative splicing isoforms from the data of RNA seq?

I am analyzing RNA seq data for the alternative splicing isoforms of my gene of interest, but unable to find a reliable and user friendly software/tool. Has any one (related to bioinformatics,...

05 June 2015 9,954 4 View

What is the minimum size requirement for LAMP-PCR amplification?

I want to amplify a short RNA sequence (about 170bases) but am unable to design LAMP primers as the software (Primer Explorer) gives the message that the minimun size is about 200 bases. Has any...

05 June 2015 4,843 10 View

Has any one used the Oxford Nanopore's MinION sequencer?

Recently i came to know about a mobile DNA sequencer launched by Oxford (MinION). Has any used it? what about Its efficiency and the reagents required for sequencing?

05 June 2015 1,528 3 View

How can I confirm Agrobacterium (GV3101) transformation?

I am facing problems in confirmation of transformed GV3103. Transformed bacteria is able to grow on antibiotic selections media (Rif+Gent+Kan) while the wild type was unable to grow. But colony...

03 April 2014 4,070 15 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View