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Questions related from Zeeshan Nasim
Hello everyone, I am working with Arabidopsis RNA-seq data and have used HISAT, TOPHAT and STAR aligners that gives a summary statistics showing the percentage of uniquely mapped reads and...
06 June 2018 6,468 5 View
Hello everyone, I introduced a gene tagged with GFP to Arabidopsis, its expressing at both transcript and protein level and is showing the phenotype somewhat similar to what I expected. Now i...
08 August 2016 4,299 2 View
I want to amplify a short RNA sequence (about 170bases) but am unable to design LAMP primers as the software (Primer Explorer) gives the message that the minimun size is about 200 bases. Has any...
06 June 2015 4,895 10 View
I am analyzing RNA seq data for the alternative splicing isoforms of my gene of interest, but unable to find a reliable and user friendly software/tool. Has any one (related to bioinformatics,...
06 June 2015 10,005 4 View
Recently i came to know about a mobile DNA sequencer launched by Oxford (MinION). Has any used it? what about Its efficiency and the reagents required for sequencing?
06 June 2015 1,577 3 View
Can any one recommend an efficient protocol for the transformation of B. juncea using Agrobacterium (GV3101)?
05 May 2014 5,131 2 View
I am facing problems in confirmation of transformed GV3103. Transformed bacteria is able to grow on antibiotic selections media (Rif+Gent+Kan) while the wild type was unable to grow. But colony...
04 April 2014 4,155 15 View
