I am trying to perform an assay to quantify peroxisomal beta-oxidation activity in liver homogenates. I've tried to follow a similar protocol that he authors of this article did, based on luminometric detection of the H2O2 produced in peroxisomal beta-oxidation: Article Reduction of n-3 PUFAs, specifically DHA and EPA, and enhanc...

However, I am having some issues and I'm not able to detect activity in the homogenates, although the system is capable of detecting H2O2 concentrations of 0,2-0,4 uM. The protocol in the paper is a bit confusing, asit is not clear the order of the components to be added to the assay mixture.

Does anyone have a detailed protocol to detect this activity in homogenates by luminometry/fluorescence/spectophotometry...?

Thanks in advance,

Joan